High-Throughput Light Scattering Tools for Characterizing and Formulating Macromolecules and Nanoparticles

11:50 - 12:20


        From aggregates to conjugates and peptides to polysaccharides, light scattering detectors combine with multiple sample preparation and delivery systems to provide a comprehensive suite of biophysical characterization tools. The light scattering toolkit determines molar mass, size, charge, interactions, conformation and conjugation of macromolecules and nanoparticles. This seminar presents an overview of the instrumentation and examples of the tasks relevant to well-characterized biopharmaceuticals that may be accomplished with these tools, focusing on three central techniques: µSEC-MALS-DLS, SEC-MALS-IV and HT-DLS.

Two of Wyatt’s light scattering tools contribute major productivity enhancements in a biopharmaceutical setting, greatly increasing the speed of analysis without sacrificing sensitivity: the µDAWN and DynaPro Plate Reader II.

        • The µDAWN adds multi-angle light scattering (MALS) and dynamic light scattering (DLS) capabilities to UHPLC, for enhanced resolution and speed in absolute molar mass determination. As will be presented in this seminar, all the standard SEC-MALS-DLS applications such as aggregate, fragment and protein conjugate analysis carry over to µSEC-MALS
        • The DynaPro Plate Reader II brings dynamic light scattering (DLS) to the realm of high-throughput, automated measurements of size, eliminating tedious cuvette-based measurements. In this seminar, the applications of the DynaPro for aggregation and stability studies will be presented